Module guide

Module LS4020 D

Module part LS4020D: Microscopy: techniques and applications (StrAnaMikr)


1 Semester
Turnus of offer:

each winter semester
Credit points:

Course of studies, specific field and terms:
  • Master MLS 2018 (module part), structure biology, 1st semester
  • Master Infection Biology 2018 (module part), Interdisciplinary modules, 1st semester
  • Master Biophysics 2019 (module part), biophysics, 1st semester
  • Master CLS 2016 (module part), MML with specialization in Life Science, 3rd semester
  • Master MLS 2016 (module part), structure biology, 1st semester
  • Master Infection Biology 2012 (module part), Interdisciplinary modules, 1st semester
  • Master CLS 2010 (module part), computational life science / life sciences, 3rd semester
Classes and lectures:
  • Microscopy: techniques and applications (lecture, 2 SWS)
  • 60 Hours private studies
  • 30 Hours in-classroom work
Contents of teaching:
  • Light microscopy
  • Confocal microscopy
  • 2-photon microscopy
  • Light sources and detectors
  • Fluorescent Dyes; GFP and genetically encoded fluorescence markers; Live Cell/tissue imaging: considerations/limitations
  • Labelling/identifying cell components using fluorescence techniques
  • Protein-protein Interactions in living cells: FRET, FLIM; Biosensors
  • Photo-activatable/-switchable Fluorescent Proteins; Fluorescent Timers
  • Advanced 3D-Fluoresence Microscopy, STED, PALM, STORM
  • In vivo imaging in tissues and living animals
  • Applications of Flow Cytometry & Fluorescence-activated Cell Sorting
  • Electron Microscopy: TEM, Immunogold label; Survey of cell ultrastructure; Correlative EM/light microscopy; Scanning Electron Microscopy (SEM)
  • Bioluminescence; high-content screening; outlook: emerging technologies
  • Data storage/formats; Course discussion; and then: “Cinema of the Cell”
  • The students are able to explain the basics of light and fluorescence microscopy and electron microscopy.
  • They know the methods for labelling and visualization of proteins and subcellular compartments in detail and are able to plan the experiments.
  • They know how to implemet live cell imaging, in vivo imaging and quantitative fluorescence techniques into biological researchs.
Grading through:
  • see Notes
Responsible for this module:
  • Siehe Hauptmodul
  • -:
  • -:
  • -:
  • offered only in English

Is module part of:
- LS4021-KP06 (former LS4020-IB) -> Prof. Hübner
- LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 -> Prof. Peters

For Master MLS with specialization in Structure Biology the module is mandatory.

(Contribution to lecture, Biology 60%)
(Contribution to lecture, Biomedical Optics 40%)

Letzte Änderung: