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Module LS4020 D

Module part LS4020D: Microscopy: techniques and applications (StrAnaMikr)

Duration:
1 Semester
Turnus of offer:
each winter semester
Credit points:
3
Course of studies, specific field and terms:
  • Master MLS 2018 (module part), structure biology, 1st semester
  • Master Infection Biology 2018 (module part), Interdisciplinary modules, 1st semester
  • Master Biophysics 2019 (module part), biophysics, 1st semester
  • Master CLS 2016 (module part), MML with specialization in Life Science, 3rd semester
  • Master MLS 2016 (module part), structure biology, 1st semester
  • Master Infection Biology 2012 (module part), Interdisciplinary modules, 1st semester
  • Master CLS 2010 (module part), computational life science / life sciences, 3rd semester
  • Master MLS 2009 (module part), structure biology, 1st semester
Classes and lectures:
  • Microscopy: techniques and applications (lecture, 2 SWS)
Workload:
  • 60 Hours private studies
  • 30 Hours in-classroom work
Contents of teaching:
  • Light microscopy
  • Confocal microscopy
  • 2-photon microscopy
  • Light sources and detectors
  • Fluorescent Dyes; GFP and genetically encoded fluorescence markers; Live Cell/tissue imaging: considerations/limitations
  • Labelling/identifying cell components using fluorescence techniques
  • Protein-protein Interactions in living cells: FRET, FLIM; Biosensors
  • Photo-activatable/-switchable Fluorescent Proteins; Fluorescent Timers
  • Advanced 3D-Fluoresence Microscopy, STED, PALM, STORM
  • In vivo imaging in tissues and living animals
  • Applications of Flow Cytometry & Fluorescence-activated Cell Sorting
  • Electron Microscopy: TEM, Immunogold label; Survey of cell ultrastructure; Correlative EM/light microscopy; Scanning Electron Microscopy (SEM)
  • Bioluminescence; high-content screening; outlook: emerging technologies
  • Data storage/formats; Course discussion; and then: “Cinema of the Cell”
Qualification-goals/Competencies:
  • Basics of light and fluorescence microscopy and electron microscopy
  • Detailed knowledge of methods for labelling and visualization of proteins and subcellular compartments
  • Applications of live cell imaging, in vivo imaging and quantitative fluorescence techniques
Grading through:
  • see Notes
Responsible for this module:
  • Siehe Hauptmodul
Teachers:
  • Prof. Dr. rer nat. Rainer Duden
Literature:
  • -: http://micro.magnet.fsu.edu/primer/index.html
  • -: http://www.microscopyu.com/smallworld/
  • -: http://www.olympusmicro.com/
Language:
  • offered only in English
Notes:

Is module part of: - LS4021-KP06 (former LS4020-IB) -> Prof. Hübner - LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 -> Prof. Peters For Master MLS with specialization in Structure Biology the module is mandatory. (Contribution to lecture, Biology 60%) (Contribution to lecture, Biomedical Optics 40%)

Letzte Änderung:
17.7.2019