Website
Module guide

Module LS4020 A

Module part LS4020A: Crystallography (StrAnaKris)

Duration:


1 Semester
Turnus of offer:


each winter semester
Credit points:


3
Course of studies, specific field and terms:
  • Master CLS 2023 (Module part of a compulsory module), MML with specialization in Life Science, 3rd semester
  • Master Infection Biology 2018 (Module part of a compulsory module), Infection Biology, 1st semester
  • Master Infection Biology 2012 (Module part of a compulsory module), Infection Biology, 1st semester
  • Master Biophysics 2019 (Module part of a compulsory module), biophysics, 1st semester
  • Master CLS 2016 (Module part of a compulsory module), MML with specialization in Life Science, 3rd semester
  • Master MLS 2018 (Module part of a compulsory module), life sciences, 1st semester
  • Master MLS 2016 (Module part of a compulsory module), life sciences, 1st semester
Classes and lectures:
  • LS4021-V: Crystallography (lecture, 2 SWS)
Workload:
  • 30 Hours in-classroom work
  • 60 Hours private studies
Contents of teaching:
  • Crystal growth, precipitant and phase diagram, crystal morphology, symmetry and space groups, crystallogenesis
  • X-rays, X-ray sources, X-ray diffraction, Bragg's law, reciprocal lattice and Ewald-sphere construction
  • X-ray diffraction by electrons, Fourier analysis and synthesis
  • Protein structure determination by X-ray diffraction, crystallographic phase problem, Patterson map, molecular replacement (MR), multiple isomorphous replacement (MIR), multi-wavelength anomalous diffraction (MAD)
  • Crystallography and the drug discovery process: studying protein-ligand interactions
  • Practical exercises employing an X-ray generator (collection of a diffraction image) and the computer (MR; calculation and interpretation of electron density maps)
  • Site visit at the Synchrotron DESY (Hamburg)
Qualification-goals/Competencies:
  • They have a general scientific competence in macromolecular X-ray diffraction analysis
  • They have the methodological competence to grow protein crystals by hanging or sitting drops
  • They have the methodological competence to correctly interpret (salt or protein) the diffraction image of a crystal using the Ewald Sphere construction
  • They have the methodological competence to tackle the phase problem either by MR, MIR or MAD
  • They can calculate and interprete electron density maps
  • They have the methodological competence, to apply structure- or fragment-based techniques for lead compound identification
  • They have the communication competency to convey the principles of X-ray diffraction theory
Grading through:
  • see Notes
Responsible for this module:
  • Siehe Hauptmodul
Teachers:
  • Dr. math. et dis. nat. Jeroen Mesters
Literature:
  • Jan Drenth: Principles of Protein X-ray Crystallography - Science+Business Media, LLC, New York
Language:
  • offered only in English
Notes:

Is part of Module:
- LS4021-KP06 (former LS4020-IB) -> Prof. Hübner
- LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 -> Prof. Peters
- LS4026-KP06 start in 2023

4 exercises, 2 hours each, are offered in addition to the lecture. Dates are given at the start of the semester.

It is a compulsory module part for the Master MLS with a focus on structural biology.

Letzte Änderung:
17.2.2022