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Module LS4020 A

Module part LS4020A: Crystallography (StrAnaKris)

Duration:
1 Semester
Turnus of offer:
each winter semester
Credit points:
3
Course of studies, specific field and terms:
  • Master MLS 2018 (module part), structure biology, 1st semester
  • Master Infection Biology 2018 (module part), Interdisciplinary modules, 1st semester
  • Master Biophysics 2019 (module part), biophysics, 1st semester
  • Master CLS 2016 (module part), MML with specialization in Life Science, 3rd semester
  • Master MLS 2016 (module part), structure biology, 1st semester
  • Master Infection Biology 2012 (module part), Interdisciplinary modules, 1st semester
  • Master CLS 2010 (module part), computational life science / life sciences, 3rd semester
  • Master MLS 2009 (module part), structure biology, 1st semester
Classes and lectures:
  • Crystallography (lecture, 2 SWS)
Workload:
  • 60 Hours private studies
  • 30 Hours in-classroom work
Contents of teaching:
  • Crystal growth, precipitant and phase diagram, crystal morphology, symmetry and space groups, crystallogenesis
  • X-rays, X-ray sources, X-ray diffraction, Bragg's law, reciprocal lattice and Ewald-sphere construction
  • X-ray diffraction by electrons, Fourier analysis and synthesis
  • Protein structure determination by X-ray diffraction, crystallographic phase problem, Patterson map, molecular replacement (MR), multiple isomorphous replacement (MIR), multi-wavelength anomalous diffraction (MAD)
  • Crystallography and the drug discovery process: studying protein-ligand interactions
  • Practical exercises employing an X-ray generator (collection of a diffraction image) and the computer (MR; calculation and interpretation of electron density maps)
  • Site visit at the Synchrotron DESY (Hamburg)
Qualification-goals/Competencies:
  • They have a general scientific competence in macromolecular X-ray diffraction analysis
  • They have the methodological competence to grow protein crystals by hanging or sitting drops
  • They have the methodological competence to correctly interpret (salt or protein) the diffraction image of a crystal using the Ewald Sphere construction
  • They have the methodological competence to tackle the phase problem either by MR, MIR or MAD
  • They can calculate and interprete electron density maps
  • They have the methodological competence, to apply structure- or fragment-based techniques for lead compound identification
  • They have the communication competency to convey the principles of X-ray diffraction theory
Grading through:
  • see Notes
Responsible for this module:
  • Prof. Dr. rer. nat. Christian Hübner
  • Prof. Dr. rer. nat. Thomas Peters
Teachers:
  • Dr. math. et dis. nat. Jeroen Mesters
  • Prof. Dr. rer. nat. Rolf Hilgenfeld
Literature:
  • Jan Drenth: Principles of Protein X-ray Crystallography - Science+Business Media, LLC, New York
Language:
  • offered only in English
Notes:

Is part of Module: - LS4021-KP06 (former LS4020-IB) -> Prof. Hübner - LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 -> Prof. Peters 4 exercises, 2 hours each, are offered in addition to the lecture. Dates are given at the start of the semester. For Master MLS with specialization Structure Biology the module is mandatory.

Letzte Änderung:
17.7.2019