Duration:
1 Semester | Turnus of offer:
each winter semester | Credit points:
6 |
Course of studies, specific field and terms: - Master Infection Biology 2023 (optional subject), life sciences, 1st semester
- Master Biophysics 2023 (Module part of a compulsory module), life sciences, 1st semester
- Master Molecular Life Science 2023 (optional subject), structure biology, 1st semester
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Classes and lectures: - Single Molecule Methods (lecture, 2 SWS)
- NMR-Spectroscopy (lecture, 2 SWS)
| Workload: - 60 Hours in-classroom work
- 120 Hours private studies
| |
Contents of teaching: | - Lecture Singel Molecule Methods: Physical basics of fluorescence
- Photo physics
- Microscopy techniques
- Protein labeling
- Fluorescence resonance energy transfer
- Single molecule enzymology
- Single molecule protein folding
- Physical basics of optical tweezers
- Protein folding with optical tweezers
- NMR-Spectroscopy:
- Assignment of NMR spectra
- Description of the NOESY experiment using the vector model Chemical Exchange and Transfer-NOEs
- Multidimensional NMR spectroscopy
- Assignment strategy for peptides
- Introduction into the product operator formalism (POF)
- Description of the COSY and of the HSQC experiment using POF
- NMR experiments for the assignment of proteins
- NMR structural analysis of proteins
- Experiments to probe the motions of protein
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Qualification-goals/Competencies: - Lecture Singel Molecule Methods:
- Understanding of the physical basics of single molecule methods
- Understanding of the benefits of single molecule methods
- Understanding of the limits of single molecule methods
- Lecture NMR-Spectroscopy:
- Students are able to assign and analyze complex NMR spectra
- Understanding of NMR experiments based on the product operator formalism
- Students are able to analyze structure and dynamics of proteins through NMR experiments
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Grading through: |
Responsible for this module: - Prof. Dr. rer. nat. Ulrich Günther
Teachers: |
Literature: - Lakowicz, Joseph R: Principles of Fluorescence Spectroscopy - ISBN 978-0-387-46312-4
- Markus Sauer, Johan Hofkens, Jörg Enderlein: Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules - ISBN: 978-3-527-31669-4
- James Keeler: Understanding NMR Spectroscopy
- Horst Friebolin: Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung
- Malcolm H. Levitt: Spin Dynamics - Basics of Nuclear Magnetic Resonance
- D. Neuhaus & M. P. Williamson: The Nuclear Overhauser Effect in Structural and Conformational Analysis
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Language: |
Notes:Is module part of: - LS4021-KP06 (former LS4020-IB) -> Prof. Hübner - LS4020-KP06 (former LS4020-MLS) and LS4020-KP12 This module part is identical to LS4020 C-MIW without seminar. Prerequisites for the module: - nothing Prerequisites for admission to the written examination: - snothing Module exam: - LS4027-L1: Bioanalytics B, written exam, 90 min, 100 % module grade(included content of LS4022-V Single Molecule Methods und LS4024-V NMR-Spectroscopy) |
Letzte Änderung: 25.3.2024 |
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