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Module guide WS 2023

Module LS4027-KP06

Bioanalytics B (BioanalyB)

Duration:


1 Semester
Turnus of offer:


each winter semester
Credit points:


6
Course of studies, specific field and terms:
  • Master Infection Biology 2023 (optional subject), life sciences, 1st semester
  • Master Biophysics 2023 (Module part of a compulsory module), life sciences, 1st semester
  • Master Molecular Life Science 2023 (optional subject), structure biology, 1st semester
Classes and lectures:
  • Single Molecule Methods (lecture, 2 SWS)
  • NMR-Spectroscopy (lecture, 2 SWS)
Workload:
  • 60 Hours in-classroom work
  • 120 Hours private studies
Contents of teaching:
  • Lecture Singel Molecule Methods: Physical basics of fluorescence
  • Photo physics
  • Microscopy techniques
  • Protein labeling
  • Fluorescence resonance energy transfer
  • Single molecule enzymology
  • Single molecule protein folding
  • Physical basics of optical tweezers
  • Protein folding with optical tweezers
  • NMR-Spectroscopy:
  • Assignment of NMR spectra
  • Description of the NOESY experiment using the vector model Chemical Exchange and Transfer-NOEs
  • Multidimensional NMR spectroscopy
  • Assignment strategy for peptides
  • Introduction into the product operator formalism (POF)
  • Description of the COSY and of the HSQC experiment using POF
  • NMR experiments for the assignment of proteins
  • NMR structural analysis of proteins
  • Experiments to probe the motions of protein
Qualification-goals/Competencies:
  • Lecture Singel Molecule Methods:
  • Understanding of the physical basics of single molecule methods
  • Understanding of the benefits of single molecule methods
  • Understanding of the limits of single molecule methods
  • Lecture NMR-Spectroscopy:
  • Students are able to assign and analyze complex NMR spectra
  • Understanding of NMR experiments based on the product operator formalism
  • Students are able to analyze structure and dynamics of proteins through NMR experiments
Grading through:
  • written exam
Responsible for this module:
  • Prof. Dr. rer. nat. Ulrich Günther
Teachers:
Literature:
  • Lakowicz, Joseph R: Principles of Fluorescence Spectroscopy - ISBN 978-0-387-46312-4
  • Markus Sauer, Johan Hofkens, Jörg Enderlein: Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules - ISBN: 978-3-527-31669-4
  • James Keeler: Understanding NMR Spectroscopy
  • Horst Friebolin: Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung
  • Malcolm H. Levitt: Spin Dynamics - Basics of Nuclear Magnetic Resonance
  • D. Neuhaus & M. P. Williamson: The Nuclear Overhauser Effect in Structural and Conformational Analysis
Language:
  • offered only in English
Notes:

Is module part of:
- LS4021-KP06 (former LS4020-IB) -> Prof. Hübner
- LS4020-KP06 (former LS4020-MLS) and LS4020-KP12

This module part is identical to LS4020 C-MIW without seminar.

Prerequisites for the module:
- nothing

Prerequisites for admission to the written examination:
- snothing

Module exam:
- LS4027-L1: Bioanalytics B, written exam, 90 min, 100 % module grade(included content of LS4022-V Single Molecule Methods und LS4024-V NMR-Spectroscopy)

Letzte Änderung:
25.3.2024