Module Guide since WS 2016/17

Modul LS2810-KP04

Stem Cell Technology (PluStamZel)


1 Semester
Turnus of offer:

each summer semester
Credit points:

Course of studies, specific field and terms:
  • Bachelor MLS 2016 (optional subject), life sciences, 4th semester
  • Bachelor MLS 2018 (optional subject), life sciences, 4th semester
Classes and lectures:
  • Stem Cell Technology Seminar (practical course, 2 SWS)
  • Stem Cell Technology Seminar (seminar, 1 SWS)
  • 45 Hours in-classroom work
  • 75 Hours private studies
Contents of teaching:
  • This seminar, offered in the cell culture labs at the Institute of Neurogenetics, provides inside and practical experience of the generation/application of human induced pluripotent stem cells (iPSC): Seminar:
  • Basics of stem cell and developmental biology
  • Basics of cell culture techniques
  • Introduction to the reprogramming of somatic cells into iPSCsBasics of cell culture techniques
  • Introduction to the differentiation of stem cells / Application for disease modeling
  • Presentation of CRISPR/Cas9 technology as a tool to genome edit iPSCs
  • Practical part:
  • Cultivation of iPSCs (Freezing, thawing, passaging)
  • Characterization of iPSCs by immunostaining and live cell assays
  • Plating and immunostaining of cortical iPSC-derived neurons followed by confocal microscopy analysis
  • Design of gRNAs for CRISPR knockout, CRISPRa, and CRISPRi
  • Presentation of a relevant publication regarding iPSC and CRISPR/Cas9 technologies in form of a 10-minute talk
  • The students know the basics of cell culture using the example of iPSCs
  • They can perform an immunostaining of cells and know how to analyze cellular structures by using confocal microscopy software
  • They can describe the basics of the new technologies iPSCs and CRISPR/Cas9
Grading through:
  • participation in discussions
Responsible for this module:
  • Prof. Dr. Philip Seibler
  • Prof. Dr. Philip Seibler
  • :
  • offered only in German

without grades

Letzte Änderung: