Module Guide since WS 2016/17

Modul LS3150-KP10

Molecular Biology (MolBioKP10)


1 Semester
Turnus of offer:

each winter semester
Credit points:

Course of studies, specific field and terms:
  • Bachelor MLS 2018 (compulsory), life sciences, 5th semester
  • Bachelor Nutritional Medicine 2018 (compulsory), life sciences, 5th semester
  • Bachelor Nutritional Medicine 2016 (compulsory), life sciences, 5th semester
  • Bachelor MLS 2016 (compulsory), life sciences, 5th semester
Classes and lectures:
  • Molecular Biology (seminar, 2 SWS)
  • Molecular Biology (exercise, 1 SWS)
  • Practical Course Molecular Biology (practical course, 3 SWS)
  • Molecular Biology (lecture, 2 SWS)
  • 120 Hours in-classroom work
  • 180 Hours private studies
Contents of teaching:
  • Lectures: Typically, 6 coherent blocks will be lectured.
  • Genetic engineering methods: vector types and cloning strategies
  • Regulation of eukaryotic gene expression at the DNA level: transcription, RNA polymerases, histone code, and epigenetic processes.
  • Nucleic acids: non-coding RNAs, interference RNA, CRISPR-Cas9
  • Gene therapy and recombinant vaccines
  • Regulation of eukaryotic gene expression at the RNA level; differential splicing of mRNA, molecular basis of the regulation of splicing and mRNA stability as well as significance for human diseases.
  • Mechanisms of translation; functions of ribosomal proteins and their paralogs, specialised ribosomes and diseases caused by changes in the translational machinery.
  • Exercises: Reading of scientific articles and oral presentation
  • Understanding scientific contexts
  • English as lingua franca in science
  • Practical course (groups of 2): Handling DNA and RNA; isolation, purification, enzymatic cleavage and gel electrophoretic presentation of DNA/RNA fragments.
  • Detection of gene expression at the mRNA level, ligation, transformation and selection of clones due to antibiotic resistance.
  • Prokaryotic expression of a protein fragment, and its analytical identification and preparative isolation (affinity purification)
  • Design of PCR-primers; specialized PCR techniques and identification of PCR products by electrophoresis
  • Exercise (groups of 4): Dealing with databases, use of molecular biology computer programs, creation of restriction maps
  • Computer-aided sequence analyses
  • Students are able to present basic steps of genetic engineering
  • They can explain basic mechanisms of gene expression
  • They are able to formulate basic mechanisms of RNA-regulated biological systems
  • They can present examples for the relationship between pathophysiological processes and their molecular basis
  • They are able to explain principles of gene therapy
  • They acquire the competence to handle english literature and to present it in a scientific oral presentation
  • lab course: They have skills in basic molecular-biological techniques
  • lab course: They have the basic knowledge of safety at work in molecular-biological labs
  • lab course: They know the basics of scientific documentation techniques and can work in a team
  • Basic skills to design and perform their own experiments
  • Internship: They have basic knowledge of occupational health and safety in molecular biology laboratories
  • Internship: They have the ability to document data correctly and work in a team
  • They have the basic ability to experiment independently and autonomously
  • They will develop additional skills in Digital Molecular Biology.
Grading through:
  • written exam
Responsible for this module:
  • Prof. Dr. rer. nat. Norbert Tautz
  • Dr. rer. nat. Olaf Isken
  • Prof. Dr. rer. nat. Norbert Tautz
  • PD Dr. rer. nat. Christina Zechel
  • Dr. rer. nat. Rosel Kretschmer-Kazemi Far
  • Dr. rer. nat. Sandra Schumann
  • Alberts et al.: Molecular Biology of Cells - Garland Science
  • Lodish et al.: Molecular Cell Biology - Freeman
  • Buchanan et al.: Biochemistry and Molecular Biology of Plants - Wiley Verlag
  • Watson et al.: Molekularbiologie - Pearson Studium
  • : Course script
  • offered only in German

Admission requirements for taking the module:
- None

Admission requirements for the practical course:
- Passed module LS2000-KP10 Biochemistry 1 or LS2510-KP10 Biochemistry 2

Admission requirements for participation in module examination(s):
- Successful completion of tests in the practical course during the semester

Module examination(s):
- LS3150-KP10: Molecular Biology, written exam, 90min, 100% of the module grade

(Share of Institute for Virology and Cell Biology in S is 50%)
(Share of Clinic for Neurosurgery in S is 25%)
(Share of Institute for Medical and Marine Biotechnology in S is 25%)
(Share of Institute for Virology and Cell Biology in V is 60%)
(Share of Clinic for Neurosurgery in V is 40%)
(Share of Institute for Virology and Cell Biology in practical course is 100%)
(Share of Institute for Virology and Cell Biology in practise is 100%)

Letzte Änderung: