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Module Guide since WS 2018/19

Modul LS3250 A

Part of module LS3250 A: Tissue Engineering (TissEn)

Duration:


1 Semester
Turnus of offer:


each winter semester
Credit points:


5
Course of studies, specific field and terms:
  • Bachelor MLS 2018 (Module part of a compulsory module), life sciences, 5th semester
  • Bachelor MLS 2016 (Module part of a compulsory module), life sciences, 5th semester
  • Bachelor MLS 2009 (Module part of a compulsory module), life sciences, 5th semester
Classes and lectures:
  • Tissue Engineering (lecture, 2 SWS)
  • Tissue Engineering (seminar with practical exercises, 2 SWS)
Workload:
  • 90 Hours private studies
  • 60 Hours in-classroom work
Contents of teaching:
  • Lectures: Mamalia cells in their natural environment and under in vitro culture as an example of industrial application.
  • Aging of cells in vitro
  • Established cell lines
  • Diverse in vitro culturing conditions
  • Proliferation and differentiation under in vitro conditions
  • Stem cell biology
  • Materials for medical applications
  • Fermentors, bioreactors and protein purification
  • Home work e. g. “Tissue transplantation and rejection”
  • Practical course (in groups of 2): Principles of aseptic manipulations, working in sterile containments, object and selfprotection, use of autoclaves
  • Preparation of sterile media, additives and other reagents
  • Slicing of tissue samples, transfer into tissue culture flasks for explant cultures
  • Microscopy and documentation of growing cells
  • Cell count, passaging by trypsinisation
  • Viability test, freezing of cells and reseeding after thawing
  • Adherence of cells to various matrices
  • Immunohistochemistry of intracellular and extracellular proteins
Qualification-goals/Competencies:
  • Students are able to explain principles of cell- and tissue culture to generate biocomposites from differentiated and pluripotent cells
  • They are able to explain basic principles of pro- and eukaryotic gene expression systems
  • They are able to explain basic principles of matrix biology
  • They can reproduce the aspects of stem cell biology
  • They acquire the ability to assess ethical aspects of tissue engineering
  • They improve their competence for correct documentation (within regards to the rules of GSP of the UzL) and team working skills
Grading through:
  • written exam
Responsible for this module:
  • Prof. Dr. rer. nat. Jürgen Rohwedel
Teachers:
  • Dr. med. Dipl. Biol. Judith Sewing
  • Prof. Dr. med. Jürgen Brinckmann
  • Prof. Dr. rer. nat. Dagmar Willkomm
  • Dr. rer. nat. Heyke Diddens-Tschoeke
  • Prof. Dr. rer. nat. Jürgen Rohwedel
  • Dr. rer. nat. Daniel Hans Rapoport
  • Prof. Dr. med. vet. Jennifer Hundt
  • Prof. Dr. med. Ralf Ludwig
Literature:
  • Lanza, Langer, Vacanti: Principles of Tissue Engineering
Language:
  • offered only in German
Notes:

Knowledge in Cell biology is a prerequisite for this course. Entrance requirement for the seminar with practical parts: certificate of the course Biochemistry 1 or 2.

See module LS3250-KP05

Letzte Änderung:
14.4.2021