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Module Guide since WS 2018/19

Modul LS3500-KP05, LS3500

Introduction into Structural Analysis (EinStruA05)

Duration:


1 Semester
Turnus of offer:


each summer semester
Credit points:


5
Course of studies, specific field and terms:
  • Bachelor MLS 2018 (compulsory), life sciences, 6th semester
  • Bachelor Biophysics 2016 (compulsory), life sciences, 6th semester
  • Bachelor MLS 2016 (compulsory), life sciences, 6th semester
Classes and lectures:
  • Introduction into Structural Analysis (seminar / exercises, 2 SWS)
  • Introduction into Structural Analysis (lecture, 2 SWS)
Workload:
  • 60 Hours in-classroom work
  • 90 Hours private studies
Contents of teaching:
  • Part A: Protein structure analysis by crystal X-ray diffraction:
  • Crystal growth: precipitant and phasediagram
  • Crystal morphology: symmetry and space groups
  • X-ray diffraction: Bragg's law, reciprocal lattice and the Ewald-sphere construction
  • Phase determination: Patterson map and molecular replacement
  • Part B: Basic NMR spectroscopy for the investigation of biomolecular structures: Basics of NMR spectroscopy: NMR experiments, Spin systems, the classical vector model
  • The nuclear Overhauser effect
  • Identification and characterisation of protein-ligand interactions: The transfer nOe, the STD-NMR-experiment, the HSQC experiment, the cross-saturation experiment
  • Building blocks for NMR experiments
  • Part C: Basics of mass spectrometry: Indroduction and basics
  • Ion sources and their fields of application
  • Mass analysers
  • Structural analysis of biomolecules
Qualification-goals/Competencies:
  • The students will acquire basic skills in selected biophysical techniques to analyze the structure and dynamics of biological macromolecules. The emphasis is on understanding the concepts behind these techniques.
  • Furthermore, the students will learn how to elucidate the structure of small organic molecules
Grading through:
  • written exam
Responsible for this module:
  • Prof. Dr. rer. nat. Thomas Peters
Teachers:
Literature:
  • actual papers:
  • Teil B: Horst Friebolin: Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung - Wiley-VCH
  • Alexander Mc Pherson: Introduction to Macromolecular Crystallography - 1st edition, 2003, Wiley
Language:
  • offered only in German
Notes:

Prerequisites for the module:
- nothing

Prerequisites for admission to the written examination:
- nothing

Module exam:
- LS3500-L1: Introduction into Structural Analysis, written exam, 90 min, 100 % module grade

Letzte Änderung:
13.4.2021