Modul LS2810-KP04

Workload:

• 45 Hours in-classroom work
• 75 Hours private studies

Contents of teaching:

• This seminar, offered in the cell culture labs at the Institute of Neurogenetics, provides inside and practical experience of the generation/application of human induced pluripotent stem cells (iPSC): Seminar:
• Basics of stem cell and developmental biology
• Basics of cell culture techniques
• Introduction to the reprogramming of somatic cells into iPSCsBasics of cell culture techniques
• Introduction to the differentiation of stem cells / Application for disease modeling
• Presentation of CRISPR/Cas9 technology as a tool to genome edit iPSCs
• Practical part:
• Cultivation of iPSCs (Freezing, thawing, passaging)
• Characterization of iPSCs by immunostaining and live cell assays
• Plating and immunostaining of cortical iPSC-derived neurons followed by confocal microscopy analysis
• Design of gRNAs for CRISPR knockout, CRISPRa, and CRISPRi
• Presentation of a relevant publication regarding iPSC and CRISPR/Cas9 technologies in form of a 10-minute talk