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Module Guide before WS 2016/17

Modul LS5200 SC

Module part: Structure-based design and synthesis of an antiviral compound (DDSyn)

Duration:


1 Semester
Turnus of offer:


each winter semester
Credit points:


3
Course of studies, specific field and terms:
  • Master MLS 2009 (Module part of a compulsory module), advanced curriculum MLS, 3rd semester
Classes and lectures:
  • Structure-based design and synthesis of an antiviral compound (seminar, 2 SWS)
Workload:
  • 30 Hours in-classroom work
  • 60 Hours private studies
Contents of teaching:
  • Experiment 1: Recombinant production of 3C-protease (3Cpro) of Coxsackievirus B3 in E. coli, purification of protein, activity assays
  • Experiment 2: Crystallization of 3Cpro. Conditions are chosen allowing for crystal growth in only few hours
  • Experiment 3: Collection of diffraction data of these crystals or, in case of not sufficient quality, of primed crystals
  • Experiment 4: Interpretation of electron densitiy and modelling of an inhibitor
  • Experiment 5: Practical performance of two steps of the synthesis of an inhibitor, determination of the identity of the product via ESI mass spectrometry and NMR spectroscopy
  • Experiment 6: Determination of kinetic parameters using FRET: IC50 and kkat/KM
  • Experiment 7: Working with replicons, transfection, determination of EC50 - values
Qualification-goals/Competencies:
  • This practical course offers an interdisciplinary insight in design and chemical synthesis of antiviral compounds. All practical work is performed by the students themselves. The course is based on the knowledge of the following courses: virology, biochemistry, structure analysis/crystallography, rational drug design, organic chemistry, biological chemistry
Grading through:
  • protocols
Responsible for this module:
  • Siehe Hauptmodul
Teachers:
  • Prof. Dr. rer. nat. Rolf Hilgenfeld
  • MitarbeiterInnen des Instituts
Language:
  • offered only in German
Notes:

For MLS.
Prerequisites: Passed all modules of the 1. and 2. semester MSc

Letzte Änderung:
16.4.2021